hoechst 33342 dye  (MedChemExpress)

Journal: Virulence

Article Title: Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii

doi: 10.1080/21505594.2026.2646808

Figure Lengend Snippet: Lachnospirin-1 acts on the cell membrane of AB1069. (A) Observation of the effect of lachnospirin-1 on the cell membrane under TEM. Scale: 1 µm. Red arrows indicate cell membrane rupture and content leakage; yellow arrows indicate the appearance of mesosomes. (B) Observation of PI staining fluorescence intensity under CLSM. Scale: 20 µm. (C) Quantitative analysis of PI staining. (D) Quantitative analysis of SYTOX GREEN staining. (E) Detection of membrane potential using DiSC3(5) probe. (F) Detection of membrane fluidity by Luardan staining. (G) Fluorescence labeling of FITC-lachnospirin-1 observed under CLSM, showing its localization in the cytoplasm of A. baumannii . Green fluorescence represents FITC-lachnospirin-1; red fluorescence represents the membrane stain FM4-64; blue fluorescence represents the DNA-stained Hoechst 33,342. Scale: 10 µm.

Article Snippet: FITC-lachnospirin-1 (GL Biochem Ltd., Shanghai, China) was added to the final concentration of 16 μM, and the mixture was incubated at 37°C for 1–2 h. Twenty minutes before sampling, 20 μg/mL of Hoechst 33,342 dye (MedChem Express, New Jersey, USA) was added, and 2 min before sampling, 20 μM FM4-64 fluorescent dye (MedChem Express, New Jersey, USA) was added.

Techniques: Membrane, Staining, Fluorescence, Labeling